Western blot was performed using Anti-hnRNP U Polyclonal Antibody (Product # PA5-63604) and a 110 kDa band corresponding to hnRNP U was observed in HeLa, Jurkat, NETRA-2 cl.D1, K-562 and was alos found to increase in THP-1 differentiated to macrophage upon treatment with Lipopolysaccharide in comparison to THP-1 differentiated to macrophage. Whole cell extracts (30 µg) of THP-1 differentiated to macrophage (Lane 1), THP-1 differentiated to macrophage and treated with lipopolysaccharide (100 ng/mL for 6 hours) (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), NTERA-2 cl.D1 (Lane 5) and K-562 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).